2-B-O04-5 〇小林 理美^1,2、藤田 智史²、小林 真之¹ Kobayashi Satomi^1,2, Satoshi Fujita², Masayuki Kobayashi^{1 1}日本大・歯・薬理、²日本大・歯・生物 ¹Dept. Pharmacol., Nihon Univ. Sch. Dent., ²Dept. Biol., Nihon Univ. Sch. Dent. Repetitive nociception induces ectopic pain in the orofacial area by changing local neural circuits in the insular cortex (IC). Parvalbumin-immunopositive neurons (PVNs) send abundant projections to pyramidal neurons (PNs) and strongly suppress PN activities. Therefore, potentiation of PVNs→PNs inhibitory synaptic connections in the IC may suppress ectopic pain induction. In the present study, we investigated how repetitive stimuli by optogenetics applied to PVNs change the efficiency of synaptic connections from PVNs to PNs.
First, we selectively expressed ChR2 in PVNs using Cre-loxP systems. Application of blue light to PVNs in the IC slice preparation induced action potentials in PVNs and inhibitory postsynaptic currents (IPSCs) in PNs. We investigated the protocol inducing long-term potentiation (LTP) of IPSCs. We found that optical stimuli similar to the theta burst stimulation increased to 142% of the amplitude of IPSCs. This LTP induction was independent from glutamate receptor activation. In the future, we apply this protocol to in vivo preparation to treat model animals with ectopic pain.