2-B-P-030 〇茂木 優貴、飯野 正光、金丸 和典 Yuki Motegi, Masamitsu Iino, Kazunori Kanemaru 日本大・医・生理学分野 Dept Physiol, Nihon Univ Sch Med The involvement of astrocytes in neurodegenerative diseases is attracting widespread attention in the neuroscience research field in terms of a potential therapeutic target. Astrocytic Ca²⁺ signals are affected by bioactive substances from damaged brain cells and can induce alterations of Ca²⁺-dependent cellular processes. Such alterations may lead to functional changes in astrocytes, including gene expression profiles and secretion of neuroprotective/neurotoxic molecules. Thus, analysis of astrocytic Ca²⁺ activities may provide clues to controlling neurodegenerative diseases. However, it is still unclear how the brain pathology affects astrocytic Ca²⁺ activities. Therefore, we here applied in vivo macroscopic imaging of astrocytic Ca²⁺ signals and fluorescence labeling of a disease marker protein to neurodegenerative disease model mice expressing Ca²⁺ sensor proteins. We found a spatiotemporal correlation between astrocytic Ca²⁺ signals and accumulation sites of the disease marker. Using this relationship as an indicator, we have started to develop a machine learning-assisted protocol to predict disease progression from astrocytic Ca²⁺ activities. These results and future analyses are expected to contribute to developing therapeutic strategies for neurodegenerative diseases.