2-B-P-047 〇田邉 由幸¹、松本 紗奈¹、三輪 葵¹、髙橋 南帆¹、藤田 融¹、賀川 義之²、前田 利男² Yoshiyuki Tanabe¹, Sana Matsumoto¹, Aoi Miwa¹, Minaho Takahashi¹, Yu Fujita¹, Yoshiyuki Kagawa², Toshio Maeda^{2 1}横浜薬科大・薬・薬理、²静岡県立大・薬・臨床薬剤学 ¹Lab.Mol.Cell.Pharmacol., Yokohama Univ.Pharm., ²Dept.Clin.Pharm., Univ.Shizuoka The obesity-prone ddY-H mice spontaneously develop hyperglycemia and hepatic steatosis along with a significant increase in body weight and fat mass even when fed with a normal diet, whereas the obesity-resistant ddY-L mice maintain lean and hardly develop these metabolic syndrome-like phenotypes even on a high-fat diet (HFD). To investigate differences in lipid metabolism between ddY-H and ddY-L mice, we examined whether there was any difference in fat absorption. After overnight fasting, the mice (6 weeks old) were orally administered olive oil with or without inhibiting lipoprotein lipase (LPL) by intraperitoneal injection of tyloxapol or saline. Without tyloxapol, plasma triglyceride (TG) levels increased significantly in the olive oil-administered ddY-H mice. In contrast, the TG levels in ddY-L mice remained lower; however, the TG levels in both mice were almost the same in the presence of tyloxapol. When intralipos, a soy-bean fat emulsion, was injected intraperitoneally, the increase in plasma TG levels of ddY-L mice was considerably attenuated, and again, the TG levels in both mice became the same in the presence of tyloxapol. We also found HFD-induced higher expression of LPL transcripts in epididymal fat tissue of ddY-L mice. These results suggest enhanced LPL expression may attenuate plasma TG increase in ddY-L mice.