2-B-YIA5-2 〇田邉 美奈¹、伊藤 義也¹、長田 真由子¹、山崎 拓也¹、黒田 悠¹、鎌田 真理子¹、細野 加奈子¹、畑中 公¹、馬嶋 正隆²、天野 英樹¹ Mina Tanabe¹, Yoshiya Ito¹, Mayuko Osada¹, Takuya Yamazaki¹, Yu Kuroda¹, Mariko Kamata¹, Kanako Hosono¹, Kou Hatanaka¹, Masataka Majima², Hideki Amano^{1 1}北里大・院医療・分子薬理学、²神奈川工科大学・健康医療科学部 ¹Dept. Pharm., Sch. Med., Kitasato Univ., ²Fac. Health＆Med. Sci., Kanagawa Inst. Tech. Objective: Acetaminophen (APAP) overdose causes severe acute liver failure. Impaired liver repair and regeneration after APAP hepatotoxicity leads to failed recovery and mortality. Accumulating evidence indicates that macrophages play a critical role in liver repair after APAP-induced liver injury; however, underlying mechanisms of involvement of macrophages remain unknown. Here, we examined the role of endogenous thromboxane A₂ (TXA₂) in macrophages in liver repair after APAP-induced liver injury.
 
Methods and Results: APAP (300 mg/kg, ip) was administered to macrophage-specific thromboxane prostanoid receptor (TP) deficient mice (mTPKO) and control mice (Cont). Compared with Cont, mTPKO exhibited severe liver injury as indicated by increased levels of ALT, necrotic area, and pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) and decreased expression of PCNA, a marker of hepatocyte proliferation at 48 h after APAP treatment. CD68-positive macrophages less accumulated in the livers from mTPKO, accompanied by reduced expressions of chemokines. Flow cytometry analysis revealed that the numbers of M1 macrophages in mTPKO were higher than control, while the numbers of M2 macrophages in mTPKO were lower than control. In cultured bone marrow-derived macrophages from mTPKO, M1-related gene expressions were increased and M2-related gene expressions were decreased.
Conclusions: TP receptor signaling in macrophages promoted liver repair after APAP-induced liver injury by accumulating M2 macrophages in the livers.