We investigated the effects of the synthetic flavonoid 3‘,4‘-drihydroxyflavonol on lipopolysaccharide (LPS)-induced neuroinflammatory responses in MG6 microglial cells. 3‘,4‘-Dihydroxyflavonol suppressed LPS-induced tumor necrosis factor (TNF)-α and nitric oxide (NO) production in MG6 cells. 3‘,4‘-Dihydroxyflavonol also inhibited LPS-induced phosphorylation of mammalian target of rapamycin (mTOR), which is crucial for TNF-α and NO production. LPS stimulation induced rapid phosphorylation of protein kinase B (AKT) in MG6 cells. 3‘,4‘-Dihydroxyflavonol significantly inhibited the LPS-induced phosphorylation of AKT. The inhibitory effect of 3‘,4‘-dihydroxyflavonol on TNF-α and NO production was mimicked by blockade of the mTOR and AKT pathways with mTOR inhibitor, rapamycin and AKT inhibitor, LY294002. Furthermore, LY294002 significantly inhibited LPS-induced phosphorylation of mTOR in MG6 cells. These results suggest that 3‘,4‘-dihydroxyflavonol exerts anti-neuroinflammatory effects via inhibition of the AKT-mTOR pathway in microglia.