Sepsis is a severe organ damage resulting from an uncontrolled immune response to infection pathogen. In treatment of sepsis in the early stage, antibiotics and other supportive agents are used. The animal model of sepsis have been created mainly by cecal ligation and puncture (CLP) or lipopolysaccharide (LPS) injection. However, these models have room for improvement. The objective of this study was to induce the mouse model of sepsis by intraperitoneal injection of fecal suspension and to evaluate validity of the model by application of vancomycin and prednisolone. Fecal contents in the cecal of mice were suspended in PBS, and the suspension was administered intraperitoneally to the same strain of mice. For evaluation of the usefulness of this model as a possible clinical application, vancomycin and prednisolone were subcutaneously administered after intraperitoneal administration of the cecal contents. As results, the survival rate of mice decreased in a dose-dependent manner of the fecal suspension. In the subsequent experiments, similar survival rate was obtained in the model group and treatment with vancomycin and prednisolone improved the survival rate. The model of sepsis induced by intraperitoneal administration of fecal suspension is reproducible and may be useful as an animal model of sepsis.