Extracellular vesicles (EVs) are known to play an important role in intercellular communication to deliver bioactive molecules such as proteins, nucleic acids, and lipids. Therefore, EVs are attractive research topics in the therapeutic and diagnostic areas. The foundational technology of EVs research is EVs isolation, but there are issues with reproducibility and purity. The reasons for this are,
(1) Manual operation is required for many EVs isolation methods.
(2) Low purity of isolated EVs makes stable EVs isolation difficult.
(3) It is difficult to construct a quantifiable evaluation system for isolated EVs.
　Then, we have developed a fully automated method to isolate EVs by combining an immunoprecipitation-based isolation method with reagents that minimize the influence of non-EVs components. This method enables the isolation of EVs with reproducibility, purity, and stability. Furthermore, it is also possible to quantify EVs by system for EVs marker-specific detection of isolated EVs by chemiluminescence enzyme immunoassay (CLEIA). Here we report various data obtained related to biomaterial-derived EVs using our EVs isolation method and quantification system by EVs-CLEIA.