【Background and Purpose】
We are focusing on the veterinary clinical application of Ligilactobacillus animalis isolated from healthy canine intestinal microbiota. Our previous findings have indicated that oral administration of L. animalis significantly inhibited the development of atopic dermatitis in a mouse model. The objective of this presentation is to clarify the relevance of IL-10 production from dendritic cells in the suppression of skin allergies by L. animalis.
【Methods】
L. animalis was cultured to 1×10⁹ CFU/mL according to the established method (Cultured in MRS medium for 24 hours). A mouse-derived dendritic cell line (DC2.4 cells) was co-cultured with L. animalis viable bacteria (1×10⁸ CFU/mL) for 24 hours, and the levels of IL-10 and TNF-α in culture supernatant was measured by ELISA. Phosphorylation of p65, a key transcription factor in NF-κB signaling, in dendritic cells 24 hours after co-culture with L. animalis was also detected by Western blotting.
【Results and Discussion】
Co-culture with L. animalis significantly increased the production of IL-10 and TNF-α compared to the untreated group. p65-phosphorylation was also significantly enhanced by treatment of L. animalis compared to the untreated group. Previous studies demonstrated that there is a direct or indirect relationship between stimulation of NF-κB and IL-10 production. Our findings imply that pre-treatment of L. animalis activate the NF-κB signaling and promote the IL-10 production from dendritic cells, which resulted in the inhibition of allergic diseases.