Atmospheric-Pressure Plasma devices with an operational heat close to body temperature have received considerable attention due to their great potential for a variety of biomedical applications, such as acute and chronic wound healing, and regeneration of damaged tissues. Previous studies indicate that reactive oxygen species (ROS)/reactive nitrogen species (RNS) signals indirectly generated by Atmospheric-Pressure Plasma exposure have a positive effect on wound healing. The objective of this study is to investigate the wound healing effects of Atmospheric-Pressure Plasma in a mouse model of thermal injury. Thermal injuries are a major public health problem and cause severe physiological stress. In this project, a mouse model of second-degree burn wound was generated with a solid Al bar (41 g) which preheated in boiling water (80℃) for longer than 1 min on skin in four sites of male C57/BL6 mice for 30 s. The treatments of control gas or Atmospheric-Pressure Plasma were conducted for 15~60 sec for continuous 5 days. Atmospheric-Pressure Plasma was generated by Pidi^® (Sekisui Chemical Co., Ltd.) which is the world's first therapeutic device for animals with gingivitis and halitosis using nitrogen plasma technology. Analysis will be performed on clinical signs, histological evaluations, and gene expression analysis.