Introduction
The expression of TAS2R14, histamine receptors and production were reported in oral squamous cancer cell lines. Recent study showed that TAS2Rs are expressed in several cancer cells. Roles of TAS2R14 in cancer cells remain unclear to date. The current study is to clarify the roles of TAS2R14 expressed in oral cancer cells with genome editing.
Methods
Oral cancer cell line, HSC4 subjected to genome editing of TAS2R14 showed completely TAS2R14 knock-out cell lines (TAS2R14KO). We compared the expression of Epithelial-Mesenchymal Transition associated proteins, regulators of circadian rhythm, histamine producing enzyme and receptors, and mitochondrial proteins by western blot analysis. Furthermore, to investigate the role in the cell growth, scratch assay was performed.
Results
E-cadherin and three mitochondrial proteins were significantly up-regulated in TAS2R14KO cells. On the other hand, N-cadherin, MAPK(p38), HDC, H1R, H2R, circadian rhythm regulators were significantly down-regulated in TAS2R14KO cells. The wound healing process was significantly delayed in TAS2R14KO cells.
Discussion and conclusion
TAS2R14 was involved in a variety of functions such as maintenance of epithelial phenotypes and probably, circadian rhythm regulation mitochondrial oxidative phosphorylation pathway.