Lavender essential oil (LEO) has analgesic, sedative and antianxiety effects. Recently, we reported that analgesic effect of linalool (LL), a component of the LEO is involved in the inhibition of nociceptive transient receptor potential ankyrin 1 (TRPA1) channel (Hashimoto et al., 2023). In addition to LL, linalyl acetate (LA) is also a major component of LEO, but its analgesic mechanisms have not been clarified. In this study, we investigated the effect of LA on TRPA1 channel in mouse sensory neurons and heterologously TRPA1 expressing HEK293 cells (TRPA1-HEK). To evaluate channel activity intracellular Ca²⁺ concentration ([Ca²⁺]_i) was measured by Ca²⁺-imaging system and membrane currents were recorded by whole-cell patch-clamp technique. In TRPA1-HEK, LA suppressed [Ca²⁺]_i and current responses to exogenous TRPA1 agonists, allyl isothiocyanate (AITC) and carvacrol, and endogenous one, Prostaglandin J₂ (PGJ₂). The inhibitory effects of LA on AITC and PGJ₂ were greater than on carvacrol. In mouse sensory neurons LA induced [Ca²⁺]_i increases, which were abolished by a TRPA1 antagonist, A967079 and disappeared in neurons from TRPA1-gene deficient mice. Pretreatment of LA suppressed subsequently applied PGJ₂-induced [Ca²⁺]_i responses. These results suggest that the inhibition of nociceptive TRPA1 channel is related to the analgesic action of LA. Therefore, the components of LEO may be promising compounds for the development of new analgesic drugs.