【Purpose】 To search for microRNAs that prevent cancer recurrence from exosomes in the blood of cancer patients undergoing IVR (Interventional Radiology), and to develop new nucleic acid drugs for cancer.
【Subjects】 Blood of recurrent and non-recurrent cancer patients after IVR, and healthy subjects.
【Methods】 Exosomes were extracted from the blood of cancer patients and healthy subjects, and RNA was purified. Furthermore, microRNA was detected by GeneChip miRNA 4.0 Array analysis. In addition, microRNA mimic was transfected into colon cancer cells (HCT116), and the viability of cancer cells was evaluated by Calcein/PI fluorescence staining.
【Results】 MicroRNA expression patterns were different in exosomes in the blood of recurrent cancer patients, non-recurrent cancer patients, and healthy subjects. Furthermore, we found 18 microRNAs with significantly more than 3-fold abundance in non-recurrent cancer patients. Among the 18 microRNA mimics, 3 microRNAs reduced cancer cell viability to less than 20%.
【Summary】 Since exosomal microRNA in the blood of non-recurrence-free cancer patients markedly decreased the viability of cancer cells, it is expected as a novel nucleic acid drug to prevent recurrence of cancer.