Transmembrane protein 182 (TMEM182) is known to be specifically expressed in muscle and adipose tissue. Previous reports suggest that TMEM182 plays an important role in skeletal muscle development. However, the role of TMEM182 in cardiac muscle is still unknown, and this study aims to elucidate the role of TMEM182 in the process of cardiomyogenesis. Human induced pluripotent stem cells (hiPSCs) can be converted into functional cardiomyocytes along the mesoderm lineage. Therefore, we generated hiPSCs overexpressing TMEM182 in a doxycycline-inducible manner and induced differentiation into cardiomyocytes. At day12 of differentiation, the expression of cardiomyocyte markers TNNT2 and MYH6 was significantly decreased in TMEM182-overexpressing cells. Wnt/β-catenin signaling is activated during early differentiation and repressed after mesoderm formation during cardiac differentiation. Therefore, we investigated Wnt/β-catenin signaling during TMEM182 overexpression. We found that phosphorylation of GSK-3β (Ser9) and β-catenin (Ser552) was increased during TMEM182 overexpression, suggesting activation of Wnt/β-catenin signaling. We further focused on integrin-linked kinase (ILK) as mechanisms by which TMEM182 activates Wnt/β-catenin signaling. The results showed that ILK expression was increased in cells overexpressing TMEM182. These results suggest that TMEM182 maintains Wnt/β-catenin signaling activation after mesoderm formation by increasing ILK expression and suppresses hiPSCs differentiation into cardiomyocytes.