Xerostomia is commonly observed in middle-aged and elderly patients. Since the adipose tissue infiltration is frequently observed in the parotid gland (PG) of alder animals. We hypothesized that the alteration in expression of fatty acid translocase (FAT/CD36), which facilitates fatty acids transportation, induces xerostomia through the hyposecretion in the PG. We firstly examined the CD36 expression in the three major salivary glands of male BALB/c mice. The PG expressed significantly substantial CD36 among them. In addition, the immunohistochemical analysis revealed that the CD36 protein localized in duct cells, but not acinar cells, in the PG of BALB/c mice. Then, the effect of CD36 inhibitor, sulfosuccinimidyl oleate, treatment on the salivary secretion in 48-weeks BALB/c mice was assessed. The inhibitor reduced pilocarpine (Pilo)-induced salivation. Moreover, the involvement of the PG CD36 in the salivary secretion of male 48-weeks senescence-accelerated mouse (SAM) was investigated. Compared with SAM resistant 1 (SAMR1), the Pilo-induced salivation in age-matched SAM prone 1 (SAMP1) was decreased. In addition, the protein expression of PG CD36 in SAMP1 was significantly lower than that of SAMR1. These results suggest that the CD36 in ducts of PG plays an important role in the aging-induced hyposecretion.