Primary cilia are immobile structures that extend from the surface of various cells and have been suggested to be involved in signal transduction from extracellular stimuli. Signal transduction through primary cilia has been reported to work in tissue regeneration, but the detailed mechanism remains unclear. Zebrafish has a higher regenerative capacity than mammals and is a suitable animal model for observing the tissue regeneration process. In this study, we investigated the role of primary cilia in tissue regeneration using knockout zebrafish of genes involved in the regulation of primary cilia formation. Trichoplein is localized in the basal bodies of primary cilia and works as a suppressor of primary cilia formation through activation of Aurora A kinase. KCTD17 is also involved in primary cilia formation through the degradation of trichoplein. In the zebrafish fin injury-repairing model, the trichoplein knockout fish showed higher regenerative capacity than the wild type. In this presentation, we demonstrate the role of trichoplein and KCTD17 in the process of zebrafish fin-regeneration.