Ca²⁺ channel blocker is one of the vasodilators with excellent efficacy in preserving organ blood flow, which may be partly affected by functional stiffness of the conduit arteries. In this study, we assessed effects of L-type Ca²⁺ channel blocker nifedipine and L/N-type Ca²⁺ channel blocker cilnidipine on stiffness of the aortic and femoral arterial segments (aortic β and femoral β, respectively) in anesthetized rabbits. The methodology to obtain aortic β and femoral β was essentially same as that for cardio-ankle vascular index with the VaSera devise for human. Antihypertensive dose of nifedipine (300 µg/kg, i.v.) increased the aortic β but hardly affected the femoral β. However, nifedipine decreased the femoral β in the presence of an α-adrenoceptor blocker doxazosin (1 mg/kg, i.v.). Antihypertensive dose of cilnidipine (30 µg/kg, i.v.) increased the aortic β but decreased the femoral β. These results suggest that the increment of the aortic β reflecting the stiffening of the aortic segment and the decrement of the femoral β reflecting the softening of the femoral arterial segment are common effects of Ca²⁺ channel blockers on the conduit arteries. In addition, the stiffness of femoral arterial segment is considered to be modified by vasoconstriction associated with sympathetic reflex.