Drug-induced and electroshock-induced animal models have been used in the development of antiepileptic drugs, but these models are seizure models, not epilepsy models. For the development of antiepileptic drugs, it is important to develop epilepsy models that mimic the mechanism of seizure generation in humans. Mutations in the gene encoding the nicotinic acetyl receptor (nAChR) causes autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE) in humans. In the present study, we evaluated the epileptic phenotype of CHRNA4 S284L mutant transgenic rats by measuring EEG and EMG, following the report of Zhu et al. We also evaluated the antiepileptic effects of carbamazepine and zonisamide. In addition, cognitive function, anxiety symptoms, and circadian rhythm were evaluated to assess the animals' psychiatric symptoms. As a result, we confirmed ADNFLE seizures with symptoms of "paroxysmal arousal," "paroxysmal dystonia," and "wandering" in TG rats. We also confirmed interictal discharges and confirmed that zonisamide was more effective than carbamazepine for these abnormal discharges. On the other hand, no cognitive dysfunction, anxiety symptoms, or circadian rhythm abnormalities were observed in TG rats. Although these TG rats did not show abnormal psychiatric symptoms in this study, they exhibited symptoms similar to those of human epilepsy and were responsive to drugs, suggesting that they may assist in the development of antiepileptic drugs.