Addiction is known to be caused by various compounds such as frequently used medicines, luxury goods, and illegal drugs, and there is a need to establish an evaluation system for side effects to prevent the formation of addiction. However, a method for evaluating addiction has not yet been established. In this study, we constructed an evaluation system to detect addiction-positive compounds by measuring the electrical activity of iPS cell-derived dopamine neurons using microelectrode arrays (MEAs). The dopamine neurons (iCell) and astrocytes (iCell) were co-cultured on MEA. After 35 days of culture, we conducted a chronic administration test of 10 addiction-related compounds and the results before and after the chronic administration were compared using principal component analysis (PCA). In the cumulative administration experiment using dopamine neurons, it was possible to detect different responses for addiction-positive and addiction-negative compounds acting on the same receptors. In the chronic administration test, four out of five compounds were detected for the addiction compound, and the non-addiction compound was not misclassified as an addictive compound, suggest that this evaluation method is capable of detecting the addiction compound. These results suggest that the evaluation system used in this study is useful as a screening system for addiction-positive compounds.