Few interstitial cystitis models can be used for evaluation over a long term although various animal models and evaluation methods exist for the development of novel clinical drugs for treatment of dysuria medicine.
Currently, we produced the acetic acid, hydrochloric acid or cyclophosphamide–induced cystitis models, but it is difficult to evaluate the efficacy in the interstitial cystitis model since the symptoms of pollakiuria for all the models recover in a few days. Therefore, we tried to establish a cystitis model in which frequent urination symptoms continue for a long period of time using hydrogen peroxide (HP). 
Female SD rats were used in the present study. First, we tested both the induction time of HP and the maintenance period of the model , comparing with those in the test groups of a sham group and the groups in which HP was stored in the bladder for 5, 15, or 30 minutes. The model rats were prepared by injection of 3% HP into the bladder under isoflurane inhalation anesthesia, and uroflowmetry was measured at 1, 2, 3, and 4 weeks after establishment of the model.
Results showed that since HP stored in the bladder for 5 minutes did not shorten the micturition interval and some rats died when HP was stored in the bladder for 30 minutes. Therefore, the model was prepared by storage of HP in the bladder for 15 minutes when a micturition interval was shortened. The micturition interval was found to be shorten up to 4 weeks in the uroflowmetry measurement. This model is considered useful for a long period of time for cystitis evaluation.