Chronic alcohol consumption might lead to the dysregulation of muscle protein metabolism through gut-muscle axis and liver-muscle axis. Moreover, synbiotics supplementation was considered to improve intestinal health and restore gut microbiota. The purpose of this study was to investigate the effects of synbiotics supplementation on the amelioration of muscle loss by improving intestinal health in rats with chronic alcohol feeding. Thirty 8 week-old male Wistar rats were divided into two groups, control group and synbiotics group. Control group (n=12) was given control liquid diet and distilled water and synbiotics group (n=18) was given control liquid diet and synbiotics solution (synbiotics group, 1.5g/kg BW/day) for 2 weeks respectively. At the 3rd week, control group was divided into C group (control liquid diet) and E group (ethanol liquid diet) and were given distilled water. The synbiotics group was divided into SC group (control liquid diet + synbiotics), ASE group (ethanol liquid diet + synbiotics) and PSE group (ethanol liquid diet + distilled water). All groups were isoenergetic pair-feeding during the experiment and the rats were sacrificed at the 8th week. E group showed liver damage, including increased ALT activity, plasma ammonia level, fatty change and higher CYP2E1 protein expression. Intestinal occludin and claudin-1 mRNA expression were significantly decreased and serum endotoxin level was significantly higher in E group. Muscle beclin-1 protein expression was significantly increased in E group. When compared to E group, the synbiotics supplementation groups, PSE and ASE, had lower ALT activity, fatty change and CYP2E1 protein expression in liver, higher occludin and claudin-1 mRNA expression in ileum and had lower beclin-1 protein expression in muscle when compared to E group. In conclusion, synbiotics supplementation might ameliorate the muscle protein degradation through lowering beclin-1 expression in chronic alcohol-fed rats by improving intestinal tight junction and reducing liver damage. Keywords: Alcoholic liver disease, Synbiotics, Gut-muscle axis, Liver- muscle axis