Sarcopenia, the major component of malnutrition in liver disease, is the most frequent complication in patients with alcoholic liver disease (ALD). Nearly 60% of patients with ALD suffer from varying degrees of muscle loss. However, there are no effective approaches to reverse sarcopenia in patients with ALD. This study aims to investigate whether the health-improvement factors, epidermal growth factor (EGF), can prevent endotoxiema, further directly ameliorate alcoholic sarcopenia (intestine-muscle axis), and/or alleviate alcohol-induced liver injury, then indirectly relieve alcoholic sarcopenia (liver-muscle axis) by maintaining intestinal integrity and microbiota diversity in rats. 6 week-old Wistar rats were divided into five groups (n=10) after one week acclimatization. Two were given control liquid diet and three were given EGF containing liquid diet for two weeks. Afterwards, control diet groups were divided into continually fed control diet (C) or ethanol liquid diet (E) for 6 more weeks. Similarly, one of EGF containing liquid diet groups was continually given the same (AEGF-C), another was changed to ethanol liquid diet without EGF (PEGF-E), and the other was changed to ethanol liquid diet with EGF (AEGF-E). Results showed that rats in group E have higher plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, plasmatic endotoxin and ammonia (p@lt@0.05). H@amp@E stain also indicated more hepatic fatty change and inflammatory cells. PEGF-E showed lower trend of AST and ALT activities. Furthermore, plasma TBARS and inflammatory cells accumulation are significantly decreased in PEGF-E (p@lt@0.05). Decline of cross sectional area of muscle was found in group E. mRNA expressions of autopahgic and degradation markers increased, while PEGF-E and AEGF-E reversed them. According to 16s rRNA sequencing outcomes, there were no significant differences in alpha diversity. Interestingly, principal co-ordinates analysis (PCoA) showed that gut microbiota were distinct between alcohol consumption groups (E, PEGF-E and AEGF-E) and those without alcohol (C and AEGF-C). In addition, PEGF-E showed the less differing with C group. In summary, alcohol consumption lead to significant liver injury, muscle degradation and gut microbiota changes. PEGF-E showed improvement on liver injury, trend to reverse muscle loss and balancing microbiota. Yet further researches are required for clarifying the role of EGF on muscle loss in alcohol consumption.